Invasion assays monitor cell movement in 3 dimensions though an extracellular matrix
Cell invasion is of particular concern in cancer research where invading tumor cells lead to metastasis, the deadliest aspect of the disease. The goal of any in vitro system should be to mimic an in vivo environment as closely as possible. To reflect this need, invasion assays from Platypus do not use artificial membranes, which are commonly found in multiple chamber systems.
Whether the cells are fully embedded in the matrix or overlaid with a thick gel layer, Platypus’ invasion assays allow you to monitor real-time cellular behavior in a physiological relevant micro-environment that more closely resembles in vivo. Our technology will help you obtain better predictive data resulting from your studies.
Platypus has developed robust invasion formats to accelerate your research.
Oris™ 3D Embedded Invasion allows cells to be seeded while fully enclosed within the 3D gel matrix from very start of the assay setup. It is believed that cells cultured in 3D produce different protein and gene expressions leading to important chances in cell behavior when compared to a similar 2D model.
Oris™ Pro Invasion is your bridge between the 2D and 3D world. Cells attach to the plate surface in a similar manner as the Oris™ Pro Migration Assay. After adhesion, the cells are overlaid with a thick collagen layer to allow cell to transition from a 2D culture to then begin moving into 3-dimensional invasion environment.